I would like to mimic gram negative cytoplasmic membranes. Therefore, in a first attempt, I thought of using 25%PG and 75% PE. Tm of DMPE is quite high, therefore, I thought using DOPE. But this lipid is known to form hexagonal phases. Does anyone have some experience with this?
Margarida Bastos
We use POPE/POPG with the same % of PE and PG that you want. The preparation is no problem, the usual, and POPE alone forms hexagonal phase (at ~71 C), and I think all PE lipids for hexagonal phase.
But the POPE/POPG (75:25) that we use does not form hexagonal phase, we studied that by SAXD. It only presents the transition from gel to liquid crystaline lamellar phase
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Beate Bersch
Thank you !
Did you measure the Tm from gel to liquid crystalline lamellar phase ???
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