Beate Bersch

3 Questions 6 Answers 0 Followers

Questions related from Beate Bersch

How do you pellet proteoliposomes and/or aggregated membrane protein by ultracentrifugation?

I would like to reconstitute a 2x40 kDa membrane protein into liposomes and to make transport assays. In the literature, such protocols are given and often include pelleting of proteoliposomes for...

05 May 2014 1,652 4 View

Use of Strep Tactin for (membrane) protein purification?

I feel that this method is not as straightforward as it is described by IBA and Co. I did not detect much useful information on the web, except for the manuals provided by the commercial...

17 December 2013 6,132 4 View

What do I have to pay attention to when preparing liposomes from PE/PG mixtures?

I would like to mimic gram negative cytoplasmic membranes. Therefore, in a first attempt, I thought of using 25%PG and 75% PE. Tm of DMPE is quite high, therefore, I thought using DOPE. But this...

13 November 2013 4,763 2 View