Okan Akar

3 Questions 16 Answers 0 Followers

Questions related from Okan Akar

Does anyone have experienced hanging drop method?

I've done hanging drop with A549 cells. (10 ul/20.000 cell/drop) At second day I saw a few cells have attacht on lid, behind the sphere colony. At third day they become much more cells that...

01 January 2018 5,970 12 View

What are the best conditions for sonication for ChIP assay?

I'm going to use abcam's ChIP assay protocol. At the sonication step it just talk about times. (5-10-15 min. etc.) But there's nothing about power or cycle. What do you prefer for 500-600 bp dna...

08 August 2015 4,612 4 View

Which loading control should be used for Pathscan microarray?

We' re doing Pathscan Intracellular Signaling Array from Cell Signaling. It says positive control but not mention which one. Is this Beta actin or something? Thank you for your interest.

11 November 2014 1,084 1 View