Michael Davis

5 Questions 75 Answers 0 Followers

Questions related from Michael Davis

What is the best way to remove extracellular bacteria from Salmonella infected BMDMs?

I am infecting BMDMs with GFP-expressing Salmonella. I need to remove as many extracellular bacteria as possible. In a trial experiment, we rinsed the BMDMs after 30 minutes of infection and...

09 September 2018 4,737 5 View

Can anyone suggest a cell line that is sensitive to apoptotic stimuli (such as CHX/TNFa) and easily transfectable?

I am studying a particular aspect of cell death and need to purify a tagged protein from apoptotic cells. Can anyone suggest cell lines that are (1) sensitive to apoptotic stimuli, (2) easily...

09 September 2017 1,419 5 View

Will coomassie blue inhibit antibody binding to pvdf-bound protein?

I am trying to purify a protein of interest for Edman sequencing. I have to stain the pvdf with coomassie blue for protein detection. If I don't see the expected band, I would like to probe for...

09 September 2017 5,853 4 View

Has anyone done an IP from culture supernatants?

I need to IP proteins and protein complexes from the culture supernatant. I am wondering if it is any different from IPs from cell lysates. For example, do you add the normal repertoire of...

09 September 2016 3,923 4 View

Co-transfection: Looking for paper demonstrating that multiple plasmids are taken up by the same cells.

As the title indicates, I am looking for an old paper I once saw demonstrating that co-transection results in all plasmids being taken up by transfected cells. Any help would be appreciated.

01 January 1970 9,519 1 View