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Questions related from Michael Davis
I am infecting BMDMs with GFP-expressing Salmonella. I need to remove as many extracellular bacteria as possible. In a trial experiment, we rinsed the BMDMs after 30 minutes of infection and...
09 September 2018 4,736 5 View
I am studying a particular aspect of cell death and need to purify a tagged protein from apoptotic cells. Can anyone suggest cell lines that are (1) sensitive to apoptotic stimuli, (2) easily...
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I am trying to purify a protein of interest for Edman sequencing. I have to stain the pvdf with coomassie blue for protein detection. If I don't see the expected band, I would like to probe for...
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I need to IP proteins and protein complexes from the culture supernatant. I am wondering if it is any different from IPs from cell lysates. For example, do you add the normal repertoire of...
09 September 2016 3,922 4 View
As the title indicates, I am looking for an old paper I once saw demonstrating that co-transection results in all plasmids being taken up by transfected cells. Any help would be appreciated.
01 January 1970 9,518 1 View