I have generated a lot of annotated cancer mutation data by NGS data analysis.
What different kind of bioinformatics analysis can be carried out on that annotated mutation data to get some useful and valuable E results?
I am transforming A. rhizogenes with the plasmid. After transformation colony pcr works with a single sharp band at the expected size. But when I perform PCR for the same colonies on replica/patch...
08 December 2021 8,433 3 View
I am looking for explanation in the context of NGS variant calling pipeline
27 May 2020 1,105 1 View
From total number of ovarian cancer cell lines present, for how many cell lines, sequencing data is available?
04 May 2020 3,955 2 View
Consurf server gives output in number of different ways. But I want result in following format, displayed in image? How to get this?
03 March 2020 3,284 1 View
I have used Swissmodel, MODELLER, Phyre, Robetta and I-TASSER for modelling my protein, but in the modelled structure, amino acid is not observed at a specific position to which I want to induce a...
16 January 2020 4,140 1 View
I used to get pure fractions using the NE-PER kit but lately I have been getting a lot of tubulin in my nuclear fractions even after washing the nuclear pellet thoroughly using PBS or cytoplasmic...
04 August 2019 3,635 0 View
I have created docked complex.pdbqt file using Autodock and I have uploaded that file in Pymol and visualized formed hydrogen bonds between ligand and protein, but the binding site residues of...
27 March 2019 1,780 11 View
Can anybody elaborate the energy concept of docked complex? and physics behind it?
18 March 2019 4,922 4 View
After running cufflink for two samples of RNA-Seq data, I used cuffmerge and executed the following command cuffmerge -o -g -s assemblies.txt Where assemblies.txt contains the...
11 March 2019 717 3 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View