What differences between Kirby-Bauer test and disk diffusion test? is it the same thing? Both of them use filter paper impregnated with a standard of sample extracted, then placed onto an agar plate that was previously inoculated with the test microbe. These method was found to be a simple, cheap and reproducible practical method.
https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/antimicrobial-drugs-13/measuring-drug-susceptibility-157/kirby-bauer-disk-susceptibility-test-791-6152/
Kirby-Bauer antibiotic testing (also called KB testing or disk diffusion antibiotic sensitivity testing) uses antibiotic-containing wafers or disks to test whether particular bacteria are susceptible to specific antibiotics.
Dear Azlan,
The test was first described by Smith, Laudicina & Rufo (1985) in their textbook 'Learning guides for the medical microbiology laboratory' to determine antibiotic sensitivities of clinical specimens. As mentioned by Mr Mushtaq the antibiotic is impregnated on paper disks, then placed on agar plate seeded/streaked with the organism to be tested. When I was doing my PhD and looking for ways to screen the growth inhibitory activity of my plant extracts, I read this book and decided to adopt the technique. I have eversince used this technique as an initial step in the screening of extracts for antimicrobial activity. However, the result is not conclusive. You have to further determine the MIC and MBC values. Mueller-Hinton agar was suggested by Smith et al, but you can use any other media that better suits your microbe. Good luck.
Kirby-Bauer test and disk diffusion test are the same thing. All aspects of the Kirby-Bauer procedure are standardized to ensure consistent and accurate results. But first you need to know something. There are a few committees that set the rules for the determination of the susceptibility and breakpoints for each antibiotic and for some groups of bacteria. The major groups working on susceptibility are CLSi (Clinical & Laboratory Standards Institute), EUCAST (The European Committee on Antimicrobial Susceptibility Testing), SFM (Société française de microbiologie) but there are others. They establish the values for resistance or susceptibility each year and breakpoint tables are constantly updated. You should look for it on the web. They have all the information you need.
Hope this helps. Good luck
Kirby-Bauer test and disk diffusion test are the same thing that we use in the laboratory for antibiotic susceptibility tests. All its results are identifying as a Desk Diffusion of the target tested Antibiotics on Muller Hinton Agar Plates inoculated with the patients' isolated pathogens.
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Microorganisms
- Bacteria