What difference does it make when a protein pellet is dissolved in SDS or PBS? Or does it depend on your next work - SDS PAGE or chromatography?

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Dominique Liger

Hi there,

SDS is a detergent which is denaturing for proteins whereas PBS is a buffer which has no nefast effect on its own on the protein structure.

Omar Gonzalez-Ortega

If the protein pellet comes from inclusion bodies you wont be able to dissolve it in pbs, need a denaturant

Banu Pradheepa Kamarajan

Oh! Bunches of thanks for the kind response... So I can store in SDS..