Has anyone used Pro-Touch color bands to mark raptors?

We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...

01 February 2021 6,786 3 View

Why have a specified percentage absorbance to be reached for flow test on UV Vis?

When performing a flow test the objective is to find the total amount of blank to be replaced by sample with pump. Why would a specified percentage of 100% i.e. 99.5% of that absorbance be used?

17 December 2020 7,762 3 View

Qiagen Viral RNA extraction kit showed similar results for positive and negative control. How should I proceed?

Our team is looking for viral RNA in the environment. We took swabs off of doorknobs using cotton dipped in water and then submerging that swab in viral transport media. We also wiped 1 surface...

08 June 2020 6,532 3 View

MitoSpy vs. MitoTacker?

I have a very long and rich history using MitoTracker dyes, but I had not, until recently, even heard of MitoSpy. Has anyone used this dye side-by-side with any of the MitoTracker? Is MitoSpy the...

25 November 2019 3,412 2 View

Where can I purchase syndiotactic polypropylene (sPP)?

Hello, I'm looking to purchase syndiotactic polypropylene (sPP). Most articles I find cite purchasing it from Sigma Aldrich, but they sold their last stock in 2013. MilliporeSigma has isotactic...

10 June 2019 6,852 6 View

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08 January 2019 3,512 5 View

How do you construct a crRNA for pCas9 (addgene 42876)?

Hello, I'm designing a CRISPR/Cas9 protocol for school purposes (I attend a extra project next to my study). I have to use the pCas9 plasmid (addgene #42876) for CRISPR eventually. This plasmid...

08 July 2018 5,461 1 View

Is the Pacbio Sequel or the Illumina HiSeq 2500 better for detection of alternative splicing by RNA-seq (next-generation sequencing, NGS)?

We have a biological condition that we believe decreases splicing fidelity in c elegans. In our preliminary RNA seq data using 2x50 bp, 22 million reads per sample and 5 replicates, we found only...

28 March 2018 3,160 3 View

How to reflect human lifespan in rat model?

I am looking to model different ages (in humans) using a rat model. Does anyone have any papers which describe the developmental markers in brains of rats and humans? Example) How many months...

06 March 2018 5,880 4 View

Has anyone compared zero air with nitrogen for nanoflow electrospray of peptides using 5600 with NanoSpray III source?

We are spending more than the cost of the service contract every year on buying 'zero air' cylinders for the electrospray sheath gas. We have the 5600 and 6600 with NanoSpray III sources. We...

31 October 2017 4,279 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View