We have been using the model for the last 3 years and it was reproducible enough: SE was observed in 70-80% male Wistar rats (160-180 g, P70).
But last experiments showed decrease in amount of rats that underwent SE to 10-20%.
The protocol is quite standart:
1. LiCl 127 mg/kg i.p. 18-24 hours prior to pilocarpine
2. Metscopolamine bromide 1 mg/kg s.c. 30 min prior to pilocarpine
3. Pilocarpine hydrochloride 25 mg/kg i.p. (all chemicals from Sigma)
Have anyone had such a problem?