Dear Nofar Ben Jashar

If it gives the same outcomes the deviations could be normalized (e.g: same membrane, optimized CCD, contrast, same loading, etc). On the other hand, if it showed different effects. Then, I would prefer to reconfirm by dose/time dependant, different cell lines with some positive control, and so on. So, there are different approaches to overcoming the issue.

Kind regards

AB Bayazid

There are several potential reasons why you might not be getting reproducible results in your western blot experiments with the U937 cell line. Some common causes of variability in western blot results include:

It is also worth noting that variations in western blot results can be caused by differences in the samples being tested, such as differences in the treatments applied to the U937 cell line in your experiments. In order to ensure reproducibility in western blot experiments, it is important to carefully control for all of these potential sources of variability.

few of the reasons were :

sample preparation gel loading and transfer, and exposure time and drug dosage time