What could be the reason for no protein expression in a tet-on doxycycline inducible system in mice?

21 February 2024 1 8K Report

I am treating tet on mice with doxycline (76ppm) for 2 weeks and i dont get the overexpression according to Western Blot. In rtPCR i see differences, but in protein level these differences are not reflected. What might be the issue?

Robert Adolf Brinzer

Could be increased ubiquitination/degradation of the protein.

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