Erjola Rapushi

6 Questions 6 Answers 0 Followers

Questions related from Erjola Rapushi

Why do i see expression in both R26lslrtTA/TRE and aMHCCre/R26lslrtTA/TRE mice after Doxycycline?

Hello, i am facing a problem with transgenic mice. I am using mice with inducible gene expression of a human transgene upon Doxycycline. These mice have aMHCre/R26 promoter with a floxed stop...

30 March 2024 2,601 0 View

Has anyone seen toxicity due to doxycycline food in mice?

I am treating mice with doxycycline (75ppm) to induce gene expression but i see dermatitis in both control and transgenic mice.

13 March 2024 4,902 1 View

What could be the reason for no protein expression in a tet-on doxycycline inducible system in mice?

I am treating tet on mice with doxycline (76ppm) for 2 weeks and i dont get the overexpression according to Western Blot. In rtPCR i see differences, but in protein level these differences are...

21 February 2024 8,257 1 View

Why i can’t detect thfa in thp1 macrophages supernatants after lps stimulation?

Hello! I am trying to detect TNFa in supernatans of THP1 macrophages (induced with 50ng/ml PMA) after stimulation with 0.1 ng/ml LPS for 24hrs. I am using Elisa but I haven’t managed to detect any...

26 January 2024 434 3 View

Does anyone know the timepoint of TNFa secretion in AC16 cells through LPS?

I am trying to detect TNFa in supernatant of AC16 cells using ELISA. To induce inflammation i incubate the cells with LPS (0.1 μg/ml) for 6 hrs. I havent managed to detect TNFa. Does anyone have a...

30 October 2023 3,897 1 View

How can i get cell lysates that are cultured in a 96well plate?

I want to do a drug screening assay in a 96well plate and test protein level of cytokines with elisa.

03 August 2023 2,606 2 View