I want to study the degree of de-differentiation of my chondrocytes in 2D culture and I want to know what source of fibroblasts (from skin, etc.) I should use as a control.
I used ScienCell previously to attain normal fibroblast for a study i did in cancer cells
I want to make a 500mM stock solution of oleic acid and linoleic acid in 100% ethanol, both of which have been supplied as liquids. Can somebody give the protocol as I'm very confused as to how...
11 March 2024 7,454 1 View
My sample has Tin electroplated on Copper. I want to selectively etch the Tin.
10 March 2024 6,990 3 View
I use 2% chitosan (w/v) along with 5.6% beta glycerophosphate (w/v) along with 0.1 M sodium bicarbonate. Cell concentration being 2x106 cells/ml. Previously i used MG63 cell lines and they used to...
21 February 2024 9,777 8 View
I have been trying to PCR amplify an ~3kb fragment from cDNA of THP1 macrophage to put restriction sites at both the ends so that I can use it for restriction cloning. My primers are 36bp long...
18 January 2024 9,146 2 View
material - Polyethelene
13 October 2023 7,502 0 View
I am writing one research article. I want collaboration in this article. From fruits science or vegetable science if anyone want to contribute, please contact me @9369641602.
27 September 2023 9,672 2 View
I'm trying to optimize methyl iodide, and I wanted to use Aug-cc-pVTZ-PP as the basis set. what is the keyword for this basis set? (I'm very new to theoretical calculation and I'm trying to learn...
21 August 2023 5,935 3 View
In one of the paper I read, I came across selection of score 1 bioactives for construction of network.
22 January 2023 8,912 1 View
I am developing a model for low temperature oxidation of coal , where coal is assumed to be porous medium and the air is coming in contact from one face , the surface oxidation of the oxygen been...
18 January 2023 8,967 1 View
Hello Readers, I am a University student and looking for some suggestions that I can explore for my thesis topic. I was particularly focussing on the topic of Supply Chain Resilience. Thank You in...
16 January 2023 9,327 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View