Hi to everyone!
I'm carrying out germination tests with onion seeds. Because of a high humidity is important for onion germination, I must place the seeds in Petri dishes which are plenty of water, and maintaining them closed at a temperature of around 20 °C.
Even though, as expected in a closed environment, high humidity lead to mould proliferation. In order to avoid that, I think that adding some drops of a fungicide could be a good idea. Although I know some fungicides which could be applied on onions, I'm not sure they are adequate for applying on seeds (which can be more vulnerable than mature plants) and inside of a laboratory (instead of at field).
I would be grateful if someone could suggest me a good fungicide that are suitable for onion seeds and laboratory use.
Thanks in advance for your responses!
Dear Dr. Pablo Reguera,
greetings, thank you for your email. I think the best methods is used colorx which used for cloth which have (sodium hypochlorite) for seed sterilization and this prevent the growth from any microorganisms.
kind regards
khaled
Dear Pablo Reguera
If you are having issues with fungal contamination you maintain the aseptic condition. Sterilize everything that is being used in the experiment, like filter paper, Petri plates, and distilled water, and carry out your experiment in a laminar. Sterilization of seeds with a 30-45 seconds dip in 1% NaOCl and 3 successive 30 seconds of wash on sterilized distilled water is a must to prevent any contamination.
Kind regards
Jiwan
Dear Dr. Pablo Reguera,
If you want to avoid the contamination from microorganisms, seeds were properly sterilized with 70% (v/v) ethanol followed by 2% (v/v) sterilizing agent, sodium hypochlorite (NaOCl) prevent the growth of microorganisms.
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