When BSA is added to an immunoassay it binds to sites where there is potential for non-specific binding without interfering with antibody/antigen binding. An added bonus of using BSA is that it stabilizes proteins while in solution.In simulated gastric fluid experiments, the first BSA domain was the first to become undetectable to specific monoclonal antibodies during digestion. In conclusion humoral IgG and IgA antibodies recognize the major BSA domains with different frequencies.Adding BSA to a reaction lessens enzyme loss on tube and pipette tip surfaces. BSA stabilizes enzymes in reaction. The stabilizing effects are most pronounced in overnight reactions..