Has anyone made an MTT assay with the Resistin like alpha gene?
Can anyone suggest a suitable porous support for fluorinated polymers? I would like to cast a membrane on the support using phase inversion technique. Any suggestions will be appreciated.... Inbal
23 March 2019 8,624 4 View
Hello, I going to run a Two step reverse transcriptase reaction on a virus that belongs to the ((−)ssRNA) group (Distemper virus) I want to be sure that am not mistaken. Which primer should I...
02 December 2018 4,333 2 View
Many papers address steady state modulation using EEG neurofeedback, but does anybody know of any studies modulating ERP components?
27 July 2014 2,008 3 View
We have been trying to incorporate AHA (L-Azidohomoalanine) into the cells, in comparison to normal Methionine, and following click-it reaction based on published protocols to test the...
23 April 2014 3,191 2 View
I just got the pNPG (sigma) substrate. Before getting started with the assay I will be happy to get some tips regarding the pNPG solution and storage. Should I dissolve it in water or buffer?...
17 February 2014 2,604 2 View
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24 January 2013 5,235 15 View
Hi, I'm not sure that my interpretation of the homogeneous group is correct, is there in glmm an easier way to distinguish between the homogeneous groups? (spss) Attached is a picture of my...
01 January 1970 7,223 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
