Hi,

For different applications of liposomes / nanoliposomes, total concentration of lipids is generally between 0.2 to 40 w/w percent (depending on how viscous you want the formulation / product to be).

This number is the sum of all the ingredients of your liposomes / nanoliposomes including phospholipids, sterols, antioxidants, proteins, polymers (e.g. PEG) that comprise the bilayer structure and its additives.

Thanks. So with 10ml (10g) water, you're suggesting 200mg to 4g of lipids? I'll presume the precise amount of lipids for optimal encapsulation will depend on a variety of factors, type of phospholipid used, the encapsulate ie water soluble or not, concentrations of other additives? Anything else to consider with regard to the points mentioned above? Thanks for your response

Hi,

It is based on basic chemical calculation.

For instance:

0.2 % (w/w) is when you take 0.2gr of the solute(s) and dissolve or suspend it in 100gr solvent / medium.

and:

40% (w/w) is when you take 40gr of the solute(s) and dissolve or suspend it in 100gr solvent / medium.

M. R. Mozafari Thank you this is helpful.

One thing that needs clarification.

Does Molar ratio relate to this?

For example if I made vesicles of:

Ascorbic Acid

Phoshatidylcholine

Glycerol,

a-tocopherol

at a 20:10:3:0.25 (AA:PC:Gl:E)

Would this be equivalent to 20% w/w for AA and 10% w/w% for PC, 3% w/w for glycerol etc etc?

You are welcome Tony,

No, the calculations mentioned above do not relate to Molar ratio.