10 October 2019 4 5K Report

I am determining drug recovery of liposomes, before calculation of encapsulation efficiency, against a calibration curve using UVvis spec. I would like some advice on whether this is a correct way of doing it.

I intend on dissolving 1ml of my empty liposome suspension with 3ml ACN/Methanol (2:1) and using my empty dissolved liposome as the blank. So we have a liposome suspension in a 4-fold dilution of solvents. Can I simply put this in the spec and blank it, then take 1ml of my loaded lipsome suspension, add the 3ml solvents and dilute when necessary until absorbance reads

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