What concentration should I use for Carbachol (Ach agonist) in a cell study?

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Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I use different force field for protein and ligand?

Hello, Dear! Generally, I used CHARMM36 + cgenff force field for simulaiton. But,, due to certain parts of lignad, force field cannot be generated using Cgenff. Can I use different force field...

10 July 2024 9,513 3 View

Ways to mitigate stimulation electrodes coming off?

I have a cathode electrode (5 x 5cm) on the femoral triangle and an anode electrode (5 x 10cm) on the gluteal fold. We are doing repeated high-intensity cycling followed by repeated Wingates. We...

07 July 2024 833 1 View

Is the polymer has low CTE if the polymer has the high chain packing and small free volume?

I wonder the tendency of coefficient of thermal expansion (CTE) of polymer which has different chain structure. In my opinion, CTE will be higher when the polymer materials has high interaction...

30 June 2024 570 5 View

How to solve Gaussian Convergence failure?

To whom it may concern, I'm working on optimizing the molecule using gaussian 16. However, it is suffering from the problem of convergence failure. I attempted optimization several times, but...

30 May 2024 6,373 3 View

Setting chlorine injection concentration??

I want to experiment with microbial control through chlorine disinfection. What I have on hand is 5% sodium hypochlorite solution. I want to set the chlorine injection concentration to 1, 3, 5,...

01 May 2024 3,741 0 View

Why hapolid cells show no growth after yeast tetra dissection?

Greetings to science community, I am operating Kluyveromyces marxianus yeast and want to generate haploid cells from diploid cells. However after 4 day since dissection, I found no growth of...

09 March 2024 8,360 0 View

What is the mechanism of ionization of transition metal?

can anyone give me advise about charge transfer transition of transition metal and rare earth element? and usually people see the FL and absorption, emission spectra of those materials in glass,...

08 March 2024 7,916 1 View

How to dissociate clumping cell?

H1781 cells are shown to tend to clump each other. H1781 is adherent cell and detached by using trypsin-EDTA. however, over-digestion using trypsin might provoke cell clumping. they seemed like...

03 March 2024 7,757 4 View

Optimal Approach for Repeated Measures Clinical Trials with Small Samples: GEE vs. Non-parametric Analysis - Expert Insights?

When undertaking clinical trials with limited sample size and repeated measures, the question arises: which is more advantageous—employing "Generalized Estimating Equations (GEE)" or opting for...

21 January 2024 1,357 3 View

How can I extract acetylcholine esterase enzyme from red blood cells through simplest way?

Dear Colleagues, I working on evaluation of choline esterase inhibitors activity,the problem that I don't have the enzyme. so i am trying to extract it from red blood cells of mice , rats ,rabbits...

30 April 2024 7,578 1 View

Has anyone studied the cholinergic innervations to the Lateral Habenula (LHb)?

Has anyone worked on the cholinergic innervations to the Lateral Habenula (LHb) in a rat model, and if these acetylcholine inputs to the LHb can be targeted via retrograde or anterograde tracers?

30 November 2023 5,025 0 View

Someone have a precise protocol to perform DAF-FM Fluorescence in HUVEC cells ?

We are currently having some problems with DAF2 or DAF-FM fluorescence. We use a 5µM concentration and are not able to see any effect of a stimulation on DAF fluoresnce. When looking cells under...

16 November 2023 8,182 0 View

How to explain the similarity of botulism and desminopathy (myofibrillar myopathy)?

In a patient with desminopathy (mutation Thr341Pro DES in the heterozygous state) with the progression of the disease, we note signs and symptoms that are also characteristic of botulism:...

18 June 2023 2,525 6 View

How to synthesize the neurotransmitter acetylcholine?

29 May 2023 1,469 1 View

I need to find a standard curve for acetycholinesterase (AchE)?

I am a student from a relatively developing country and the commercial enzyme is expensive for us. My graduation thesis is desperately in need of an available standard curve for my AchE assay so...

19 January 2022 2,239 4 View

In an organ bath if the volume of the bath was 10ml what is the concentration of Ach in the bath?

i have got a concentration of 10^-6M of Acetylcholine. in my organ bath, if my volume of bath was 10ml, what will be the concentration of acetylcholine in the bath if i pipette 200ul of agonist...

19 October 2021 2,015 3 View

Protocol for administration of acetycholine and norepinherphrine to cells in in vitro culture?

Hello all! I work with induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for my research. I am seeking to administer the positive chronotrope epinephrine and negative chronotrope...

29 June 2021 6,568 0 View

I was preforming the acetylcholinesterase in vitro inhibition screening and the assay didnt work. anny suggesstions?

Hello everyone, I was preforming ths assay using Ellman's method and nothing happend. I'v added Acetylcholine iodide to initiate the reaction ad prefoemd it at RT. any recommendations what I did...

19 June 2021 3,220 5 View

In the absence of Sigma products how reliable are Loba-Chemie and CDH reagents for Biochemical assays?

Good day scholars. I am not able to get some Sigma products for biochemical assays. The supplier said alternative available products are from CDH and Loba-Chemie. But people hardly refer to these...

15 June 2021 6,002 3 View