Chi Woo Yoon

7 Questions 3 Answers 0 Followers

Questions related from Chi Woo Yoon

Real-time fluorescence imaging for insulin secretion?

I am looking for the way to measure insulin secretion in real-time from single cells using fluorescence microscope. Can anybody help me with this?

23 March 2017 7,518 2 View

How do you do glucose adaptation for pancreatic beta cell lines?

I am trying to measure insulin secretion from HIT-T15 cell lines using ELISA. To culture the cell line, I am using Hank's F12K as a basal media, but it contains 10mM of glucose, and I cannot find...

09 May 2016 1,185 2 View

Is Fluo-4 am reliable to measure nuclear calcium?

I've been using Fluo-4 am (invitrogen) to image cytosolic calcium level and I noticed calcium level in nuclear is higher than cytosolic calcium. Can I compare the level of calcium between...

06 October 2015 3,149 9 View

Can BAPTA-AM be used to identify the main source of intracellular calcium elevation?

I have been doing some blocker experiments to identify the mechanism of intracellular calcium elevation from US stimulation. I used 10uM of BAPTA-AM, incubated for 20min, and wash with DPBS+. and...

12 August 2015 9,843 4 View

Can someone identify the states of the cell based on the following DAPI images?

So, basically, I am trying to see if cell is differentiating(gross changes in chromatin structure accompany cellular differentiation) based on the nuclei images, and I got a few different ways of...

24 February 2015 5,275 4 View

What is wrong in this mesenchymal stem cell culture?

Can you tell what is wrong from the following pictures?

15 January 2015 4,412 10 View

Does anyone know how to sterilze Parafilm?

I have a perfusion well-chamber with hole on it. I want to seal the hole with parafilm Do you think disinfection with 70% ethanol and UV light would be enough? through the hole parafilm will have...

07 February 2014 5,278 3 View