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Questions related from Chi Woo Yoon
I have used different recipe of physiological buffer solutions for live cell imaging previously (such as live cell imaging solution from Thermofisher). Based on Thermofisher's description, " It...
11 December 2018 2,531 0 View
I was wondering if bathing with Apyrase would affect amount of intracellular ATPs as well as extrecellular ones. Can I say Apyrase only affects extracellular ATP-ADP ratio?
27 October 2017 3,919 1 View
I am looking for the way to measure insulin secretion in real-time from single cells using fluorescence microscope. Can anybody help me with this?
23 March 2017 7,557 2 View
I have been growing hMSC on regular plastic culture dishes and wondering if there is any study has been done comparing culturing on different stiffness of substrates/matrix. I know there are many...
26 July 2016 1,750 2 View
I am trying to measure insulin secretion from HIT-T15 cell lines using ELISA. To culture the cell line, I am using Hank's F12K as a basal media, but it contains 10mM of glucose, and I cannot find...
09 May 2016 1,208 2 View
I've been using Fluo-4 am (invitrogen) to image cytosolic calcium level and I noticed calcium level in nuclear is higher than cytosolic calcium. Can I compare the level of calcium between...
06 October 2015 3,179 9 View
I have been doing some blocker experiments to identify the mechanism of intracellular calcium elevation from US stimulation. I used 10uM of BAPTA-AM, incubated for 20min, and wash with DPBS+. and...
12 August 2015 9,868 4 View
I want to test if Ach is responsible for the cell signaling that I have been looking into. I found Carbachol as agonist of Ach but couldn't find concentration info. Anybody have experience using...
03 June 2015 980 2 View
So, basically, I am trying to see if cell is differentiating(gross changes in chromatin structure accompany cellular differentiation) based on the nuclei images, and I got a few different ways of...
24 February 2015 5,301 4 View
I got mouse bone-marrow mesenchymal stem cell(passage 1) and fixed and stained them with DAPI and got following images. This is my first time dealing with mMSC but based on sizes of nucleus I...
09 February 2015 8,925 4 View
Can you tell what is wrong from the following pictures?
15 January 2015 4,439 10 View
I was growing hMSC (from Life Technologies) with alpha MEM and MSC certified FBS (both also from Life Tech.) and they were healthy for the first passage. But since early this week (second passage...
16 May 2014 4,829 26 View
I found that many people recommend to use alpha MEM for human Mesenchymal stem cell culture as basal media. Would nucleoside make any difference in stem cell culture?
14 April 2014 1,236 3 View
I have a perfusion well-chamber with hole on it. I want to seal the hole with parafilm Do you think disinfection with 70% ethanol and UV light would be enough? through the hole parafilm will have...
07 February 2014 5,305 3 View
