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Questions related from Chi Woo Yoon
I am looking for the way to measure insulin secretion in real-time from single cells using fluorescence microscope. Can anybody help me with this?
23 March 2017 7,519 2 View
I am trying to measure insulin secretion from HIT-T15 cell lines using ELISA. To culture the cell line, I am using Hank's F12K as a basal media, but it contains 10mM of glucose, and I cannot find...
09 May 2016 1,186 2 View
I've been using Fluo-4 am (invitrogen) to image cytosolic calcium level and I noticed calcium level in nuclear is higher than cytosolic calcium. Can I compare the level of calcium between...
06 October 2015 3,150 9 View
I have been doing some blocker experiments to identify the mechanism of intracellular calcium elevation from US stimulation. I used 10uM of BAPTA-AM, incubated for 20min, and wash with DPBS+. and...
12 August 2015 9,844 4 View
So, basically, I am trying to see if cell is differentiating(gross changes in chromatin structure accompany cellular differentiation) based on the nuclei images, and I got a few different ways of...
24 February 2015 5,276 4 View
Can you tell what is wrong from the following pictures?
15 January 2015 4,413 10 View
I have a perfusion well-chamber with hole on it. I want to seal the hole with parafilm Do you think disinfection with 70% ethanol and UV light would be enough? through the hole parafilm will have...
07 February 2014 5,279 3 View