Literature Review for a dissertation- any tips?

Hello everyone I hope you are all well! I just have a quick question- I am a third year BA Social Work student in the midst of writing a literature review for my dissertation. I wanted to know if...

04 January 2021 6,869 4 View

Can anyone advise me on how to amplify V4 region of fecal DNA of dss-induced colitic mice for microbiota profiling?

Animals were fed DSS via drinking water and fecal samples were collected and DNA was isolated using Qiagen stool DNA isolation kit. The V4 region of extracted DNA was amplified with specific...

20 December 2020 1,739 3 View

How do I avoid sucking up electrospun scaffolds during cell culture?

Hi, I'm a PhD student working to develop electoconductive, electrospun scaffolds for cardiac tissue engineering applications. I have been seeding cells onto these scaffolds but having major...

04 December 2020 6,672 3 View

Why is methylene blue commonly used to measure an active surface area of nanoparticles?

Among the dyes, why is methylene blue commonly used to measure an active surface area of nanoparticles(especially graphene derivatives)? I try to find the reason, but I cannot find it. Could you...

11 March 2020 2,963 4 View

Does color or lighting/reflection influence behavioral test in mice?

Our pole test apparatus includes a metal pole covered with white cotton thread, with a grey plastic base that gives a slight reflection. In our experiment, many mice reach within a few centimeters...

17 January 2020 9,969 1 View

Is there any atmosphere that Titanium chloride can make fume?

I use TiCl4 to check if there's water in the glovebox. when I opened TiCl4 there were a lot of fumes, but I never guess where the water comes. Because there was no oxygen inside. So I didn't sure...

13 January 2020 9,127 1 View

Flow panel; which cell types are identifiable?

We have begun doing experiments with human tissue and have the following markers: CD45 DAPI ------ CD3 CD4 CD8 CD11b CD11c We routinely use CD45 for lymphocytes and DAPI for live cell selection....

13 November 2019 9,106 3 View

Is there a perfect purification method for semiconductor precursor?

I tried synthesize Ethyl derivated cyclopentadienyl tris(dimethylamido)Zirconium But I have some problem in purification process I want to remove all the solvent from product (toluene) but it...

10 June 2019 4,889 2 View

Why would an RNA extract be viscous?

A student in my department has recently submitted some RNA samples to a sequencing centre for NGS. He was advised that there were problems with the library prep due to vicosity of the RNA samples....

05 February 2018 6,322 7 View

Extra band on EMSA without protein?

I am running an EMSA and keep seeing an extra band appearing without an protein. I have gel purified my FAM-labeled probe and it looks to be pure on a DNA agarose gel. I am also seeing the probe...

15 November 2017 896 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View