I am performing enzyme assay for ADH. I am confused with what concentration of NAD and Tris Cl can be used.
If any one has a protocol, please provide it for me.
See this paper:
Ka¨gi JHR, Vallee BL. 1960. The role of zinc in alcohol dehydrogenase. V.
The effect of metal-binding agents on the structure of the yeast alcohol
dehydrogenase molecule. Biol Chem 235:3188–3192.
Please refer the link below:
http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-alcohol-dehydrogenase.html
Hai Nilesh Kumar.I just saw your post when I came through this Research gate.Can you please elaborate me about your research on which plants or organisms you have done the ADH enzyme assay.
Hi Shephrou Helena
I have done this assay for Kluyveromyce marxianus to estimate the activity of alcohol dehydrogenase assay for pentose sugars.
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