Twice the concentration used for human blood, since the hemostasis system of mice is pretty procoagulant when compared with the human one.

Thank you. My collection tubes are already EDTA coated microtainers for collecting human blood, how much EDTA solution should I add for 1ml mouse blood?

1.6 mg K3-EDTA per ml blood.

General procedure I'm used to go by is as follow:

Place 5 µl of 0.17M EDTA solution into the tapered end of a Natelson blood collecting tube. Hold tube near the tapered-end between thumb and forefinger, and then flick your wrist so that the EDTA coats the entire tube (It's OK if some EDTA ejects out of the tube). Use this tube to collect blood, and then drain the blood into a Microtainer tube.

Hope that helps. 

Best,

Andre

If you intended to perform experiment with RBC,  collect blood with 5mM EDTA or CPD .

If your target is WBC, collect blood with 500mM EDTA .

Best regards, 

Babu

Twice the concentration used for human blood, since the hemostasis system of mice is pretty procoagulant when compared with the human one.