What companies can offer homologous arms to clone it in my donor vector ?

More Ahmed Mohamed Abdelbaki's questions See All

I have get this error when i calculated the geometrical optimization for prco3, it takes 12 hours until gives this message in the outputfile?

Fatal error in MPI_Allreduce: Other MPI error, error stack: MPI_Allreduce(1628)......: MPI_Allreduce(sbuf=000002459254A180, rbuf=000002459F86A140, count=4851, MPI_DOUBLE_COMPLEX, MPI_SUM,...

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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09 August 2024 9,621 0 View

How to set the environment variable for incorporating the GD3 dispersion in G16 for performing calculations using the OPBE functional?

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05 August 2024 1,494 2 View

How prion can cause disease?

how is prion can cause disease

01 August 2024 250 1 View

Has anyone used Jump 2 before?

Jump 2 is the 1st app scientifically developed to measure your jump height.

31 July 2024 8,194 0 View

Help on understanding the implementation of Mori Tanaka method on MATLAB?

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What are the most PROBLEMS of HYBRID nanofluid in oil industry ?

explain your idea

23 July 2024 1,197 0 View

Can anybody provide me the Matlab code to plot the attached picture (Time-Frequency Domain), please?

Time-Frequency Domain

19 July 2024 8,031 2 View

"Hello, I am trying to find public datasets containing FTIR spectra of blood samples (both healthy and disease-related)?

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17 July 2024 6,519 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

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How can we now if the promotor in the vector is well?

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MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

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Why knockouts for same genes from two different gRNA are showing different result?

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Illustra™ MicroSpin™ G-25 columns what it is used for?

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Pink bacterial colonies?

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What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

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22 July 2024 9,160 2 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

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The best source for amplification of ADAM17 prodomain?

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21 July 2024 8,660 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

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17 July 2024 3,371 0 View

Panduranga Rao

Hi,

it is not clear what is required? can you elaborate?

Lalhaba Oinam

Thermofisher can synthesise your custom oilgos just input your donor oligos and they will deliver to you in a jiffy and I think most primer synthesising company have this options. Check this website:

https://www.thermofisher.com/jp/ja/home/products-and-services/product-types/primers-oligos-nucleotides/invitrogen-custom-dna-oligos.html

Ed Davis

GeneCopoeia also offers HDR donor cloning services for CRISPR. Visit http://www.genecopoeia.com/product/crispr-cas9/ to learn more.

Joanne Worobec

MilliporeSigma offers a service that includes experimental analysis, gRNA validation, donor design and synthesis. I have attached an application or for more information please contact [email protected].