8 Questions 4 Answers 0 Followers
Questions related from Ahmed Mohamed Abdelbaki
for CRISPR CAS9 experiment
20 April 2016 1,206 4 View
I confused in designing homologous arm for Knock in experiment using crispr cas 9 . Does people use BAC DNA for human genome sequence and how can i buy BAC DNA for my desired sequence?
16 April 2016 7,319 3 View
I washed by QC buffer and I put the eluted buffer and forget to remove the washing buffer from the tube? Now I have tube having washing buffer and eluted with DNA. How can I solve this problem?
08 May 2015 8,711 3 View
Why Trypsin is inhibited by serum during cell culture?
16 April 2015 8,157 2 View
I try to grow MEME having 1% pencilin/ streptomysin and 10% FBS The growth of this cell very slow Did any one grow TNBC MBA-MD-436 ? Please suggest media and how to grow them i try to grow MEME...
13 April 2015 1,425 3 View
How can the cell attach to cell culture plate? I know there are two reasons. First, electrostatic reaction second, extracellular matrix but need more explanation.
11 April 2015 4,437 4 View
kuramochi -ovcar - ovarsha- HEYA8- SKVO3- OVAMANA Please help me to learn more about these cell lines.
17 November 2014 7,105 2 View
I'm working now on western blot and trying to detect Kifc1, but it gives me a dark background and I washed for 2 hrs and used 5% BSA-TBST blocking. The same problem of high background is very dark.
07 November 2014 6,421 4 View
