I cultured normal Hela cells. I subcultured them one day, then found some dots in the media the next day. Then I saw the dots are more and more, among the media and in the body of the cell, and cells begin to disassemble and die. The media during this progress is clear, so I think it is not bacteria contamination. I tried to centrifuge the cells, but I couldn't remove the dots, it is kind of sticky.
Hello,
From what you mention and from how the photo looks, it is very likely that it is bacterial contamination. What you can do is take a drop (with a serological pipette) from the media of your flask and put it on a slide and put a coverslip on it and look at it directly under the microscope. You can see if it is bacterial or yeast contamination.
Best.
Okay,
maybe the cells got stressed, check the media you use or growth factors.
Best.
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