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Questions related from Maria Fernanda Rosado
Hello, everyone Do you know some techniques to improve the efficiency of reprogramming if I use a kit (cytotune-ips 2.0 sendai vector) expired in July 2021? Thank you for your answers!
26 March 2024 7,698 4 View
Hello everyone, I have established a primary culture of fibroblasts from a human skin biopsy, how long does it take to see the fibroblasts? today is the 17th day in cultivation and I still don't...
21 August 2023 9,829 7 View
Hello, Can anybody help me??... A sendai virus kit (already used) for reprogramming has been stored at 4°C (ideally would be -80°C) for about a year, I want to use that kit, but I don't know if...
20 April 2023 7,173 4 View
Has it happened to you that fibroblasts have that morphology? I have cultivating fibroblasts, they changed morphology after one passage
31 March 2023 7,321 0 View
Hello! Has it happened to you that the fetal bovine serum is contaminating and eventually (not immediately) your cells become contaminated? If so, what did you do to fix the problem? Thank you!!!
23 March 2023 2,048 5 View
primary fibroblast culture, I am doing a primary culture of fibroblasts from skin explants, which confluence is considered to make the pass 1. confluence under the microscope 2. the confluence...
07 January 2023 8,462 7 View
