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Questions related from Mena Motaz
I did three PCR for the same DNA sample. The first and second one gave me a clear band band. However, in the last PCR I put the tubes in the PCR machine before it starts the heating cycle (25 °C)....
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After I make cDNA, I run an gel electrophoresis, but its band appears degraded. What can be the issue?
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I am little bit confuse between the composition and function of Nuclease-Free Water and Rnase-Inhibitor in RNA extraction .Does Nuclease-Free Water act as Rnase inhibitor ?
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Can I use zymography to detect the difference in enzyme activity between biological sample? AS far as I know, we use SDS in zymography to separate the protein in non-reducing condition, and then...
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