Reasons for upper corner background images in western blot ECL?
be carrefull when handling the pen you used to label the membrane... traces of ink on your finger (or gloves!) can also be a problem.
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
When we used LNP to deliver GFP mRNA to Jurkat cells, the efficiency was as high as 90%. However, when we delivered GFP mRNA to T cells, we failed, with an efficiency of 0.5-0.6%. Could anyone...
06 March 2024 3,341 2 View
Preferably the effect in the attached file can be achieved.
10 October 2023 5,661 1 View
The slow growth of H9C2 cells and the formation of cell clumps after centrifugation and resuspension might be caused by what factors?
20 August 2023 114 0 View
Dear All, I got a HAADF image of strained SrTiO3 films with ferroelectricity detected by PFM. Now I want to analyze the ferroelectric domains in the HAADF image. Can anyone tell (or share if...
09 July 2023 5,619 0 View
We assessed the ability of 50 age-matched insect lines/populations from 30 diverse geographic regions to survive infection. Median lethal times (LT50) were monitored using five replicates (20-30...
06 March 2023 913 0 View
ACGAN can generate the corresponding target image according to the tag, and its discriminator acts as the tag identification and authenticity judgment in this process. Can a good ACGAN...
09 November 2022 2,556 2 View
I have used the Cdh16-cre mice hybridizing with the X gene-flox mice to generated a tubular epithelial cells-specific X gene deletion mice. There was no problem with the genotyping, the cre was...
04 September 2022 7,304 0 View
P3HT is often used as a hole transport layer in perovsite solar cell. Is the P3HT need be oxidized as a hole trasnport layer in perovskite solar cell?And what is the oxidized condition of P3HT. Is...
08 December 2021 4,632 3 View
In electrocatalytic ammoxidation, the electrolyte is usually 0.1m NH3+ 1M KOH. How is the concentration of NH3 achieved here?
21 November 2021 3,035 2 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...
15 July 2024 9,975 1 View