Using the PrimerDesign Covid19 detection kit ( https://www.genesig.com/products/10039-coronavirus-covid-19-ce-ivd ), the following curves are obtained (HEX/FAM probe with water, and with Master Mix, without any template).
What can cause it?
I think may be reagents are contaminated or u use the old stock or the reagents are not stored properly
Dear
Your Taqman qPCR amp plot appear to indicate that somehow the amplification reaction has not taken place at all. The deltaRN values are too low to be considered as true signal. This may be attributable either to faulty qPCR base reagents, improperly designed primers/probes, improper reporter/quencher dye combination, faulty thermal block or error in thermal cycle program profile.
First be sure that your primers are working. this you can check by running a conventional PCR with your qPCR primers using a well characterized DNA template as positive control, and check the presence of amplicons in agarose gel. if you have, you can also electrophorese the products from the above qPCR run, from which you have uploaded the figures.
After ruling out the all the faulty reagent factors, check the intsrument and thermal program.
Good luck
This may be attributable either to faulty qPCR base reagents, improperly designed primers/probes, improper reporter/quencher dye combination, faulty thermal block or error in thermal cycle program profile. First be sure that your primers are working
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