Removed Question by author?

This question has been removed by author.

18 November 2020 9,422 1 View

Best tools for identifying miRNA and miRNA target prediction ?

Can anyone help me to identify miRNAs from Assembled Transcriptome (multi fasta contig files around 45,000). I checked with mirBase but it has some limitations. I did a local BLAST using mature...

28 January 2020 4,454 3 View

What is the good method for cleaning tungsten fiber before coating process?

I am working on tungsten fiber coating. My first step is to remove oxide contaminants on the surface. Please suggest me some Pre-treatment methods.

19 May 2019 5,741 3 View

Why SEM has low vertical resolution than its horizontal resolution?

Basically the BSE has low resolution than the Secondary Electrons (SE). But totally SEM including FE-SEM resulting in low vertical resolution, Why?

26 May 2018 476 5 View

Can I know how to do sensitivity analysis using R for the the given data??

The 2 columns variables and values are the input to some design and I observed those remaining column values as output. Please tell me how to write R program to have sensitivity analysis for such...

08 November 2017 9,141 1 View

During Spray Deposition process, if a billet/substrate experiences stress higher than yield, does it mean that substrate material failed(not usable)?

Or is it okay for the substrate to experience such high thermal stress?

27 August 2017 6,892 2 View

How to solve non-positive volume cells mesh issue in icepak?

Exact message "Warning: The mesh contains non-positive volume cells.This must be fixed before solve." The model is made up of .igs CAD file import. It has curved surfaces. Is there a way to check...

30 July 2017 237 3 View

Is there any book recommeded for spectrum sensing methods?

06 May 2017 4,110 4 View

Any suggestions on a high background for an ELISA reading?

Dear Friends, From past many days, i am getting problems with very high background reading for ELISA. 100ng Antigen Coated Skimmed milk 5% coating buffer having 0.5% tween Rabbit serum dilutions...

20 May 2015 9,179 13 View

What is the best method for cloning long PCR fragments?

We are trying to generate an infectious clone of a plant virus called cassava brown streak virus in 3 fragments of 3-4 Kb in size (virus is ~10Kb) but have been failing to get more than one clone...

10 October 2013 9,883 15 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Why cloning of gene into pCAMBIA1304 vector is unsuccessful?

I have tried to clone a specific gene into pCAMBIA1304 vector. But all the attempts failed. I used ligation kit from both Promega Corporation and Takara Bio. However, there was one time I used...

04 February 2021 525 1 View