What can be possible reason of failing slope of 4PL curve of indirect or sandwich ELISA?

More Pragati Vanavi Makadia's questions See All

How to generate a H-bond trajectory plot from .dat file?

I have .dat file, how do I generate the hydrogen bond plot? Is any software required or I can use Excel? I attached the .dat format.

13 January 2024 548 0 View

Does anyone have experience with isolating mRNA from frozen whole blood samples stored in RNAlater?

Hi, I am currently trying to isolate RNA from whole blood. I have frozen blood samples - preserved in RNAlater stored at - 80. Samples were collected in RNA later (300 ul blood sample and 1.3 ml...

12 October 2023 267 3 View

Hi, I want to draw supercell in VESTA with two different style like space filled and wireframe. Is it possible to draw structure such way in VESTA?

13 June 2023 5,601 1 View

Why Primary cancer cells got detached from flask after 10 days of culture ?

I had cultured primary human lung cancer cells derived from needle biopsy sample after isolation. For 10 days cells were greatly healthy and good in number. I had two different cells from...

09 January 2023 2,322 1 View

I want to isolate DNA from the exosomes. Is there any optimised protocol for it or any protocol that gives high yield of DNA?

The exosomes are purified from the blood samples from the patients. I want to know a protocol to isolate DNA from these exosomes that gives high yield.

16 March 2022 178 1 View

Can I use welltory app for HRV analysis for my research study?

I want to know can mobile phones apps which use PPG be used for HRV measurements. I want to use it for my research study comprising of more 150 participants.

23 August 2021 9,301 4 View

Can anyone please guide me or suggest me some methods for isolation and identification of stemphylium and alternaria from onion foliage?

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21 March 2021 5,882 6 View

Can i isolate sufficient amount of degradation products using analytical RP-HPLC?

I am studying some degradation products and want to isolate them from parent drug, can i use analytical HPLC to do so? it will provide sufficent amount of DPs? TIA

26 February 2020 588 10 View

Can anyone help me with Hairy root induction condition it is in dark or light?

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03 September 2019 4,705 4 View

What is the meaning of 'redox neutral' in transition metal catalyzed C-H activation reaction?

It is related to the N-N/N-O bond containing directing group.

09 May 2018 9,732 8 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

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What is the weight of rotavirus A (ng)?

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29 July 2024 3,138 2 View

How do we pick data for determination of Validation Acceptance Criteria?

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23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

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15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Kaled Nather Taha

this is a great scientific-practical question because I also need to know why we used different curve fitting to meet the high precision.

4PL,5PL, linear, log, cubic supin ...

what are the criteria based on selected the curve type in ELISA ?

Feng Wang

Pipetting error? I am not sure.

OD vs mass (or conc.) is linear, and it is S curve (4PL) when converted into log or semi-log.