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Questions related from Feng Wang
Once TMB is added the color starts to become blue, but what is the best time to add stop solution? Any method to monitor it or determine it? Why? Thank you !
11 June 2023 8,933 4 View
Here is my ELISA assay. Prepare standards. Dilute samples at different dilution factors: 20, 40, 80, 160, respectively. Add spike at the same concentration. Run ELISA. Calculate non-diluted...
22 April 2023 1,681 3 View
I am testing the residual plasmid quantity using qPCR in AAV gene therapy products. The aim is to quantify the level of residual plasmid in AAV products. I prepared standards using diluted...
29 March 2023 2,656 2 View
We are testing virus titer, and have to dilute the virus stock at 1:10,000 (Dilution A) and 1:20,000 (Dilution B) to run qPCR as templates. Then convert the diluted titer to dilution corrected...
16 March 2023 8,062 4 View
I am going to isolate nuclear protein from ~30 tissues. The lysis buffer contains 10mM NaCl and 0.1% Triton X-100 which is reported with the ability to lyse cell membrane only (but not nuclear...
30 June 2016 7,644 3 View
Actually here I have 2 questions, one is for IP experiments, and another is for cell isolation. And both are about the choice of beads. Question 1: What bead is better for IP? Dynabead from...
28 April 2015 4,193 4 View
Thank you so much.
25 June 2014 1,641 9 View
I am working with mouse Immunol cells. RAW264.7? Bone marrow derived macrophages? Peritoneal macrophages? or Spleen cells? or blood leukocytes?Thanks.
28 March 2013 1,858 0 View
Does anybody has a detailed protocol?
11 January 2013 6,107 14 View
