Feng Wang

11 Questions 59 Answers 0 Followers

Questions related from Feng Wang

How to determine the best timing of stop solution addition in ELISA?

Once TMB is added the color starts to become blue, but what is the best time to add stop solution? Any method to monitor it or determine it? Why? Thank you !

11 June 2023 8,982 4 View

How to determine the real concentration data in ELISA?

Here is my ELISA assay. Prepare standards. Dilute samples at different dilution factors: 20, 40, 80, 160, respectively. Add spike at the same concentration. Run ELISA. Calculate non-diluted...

22 April 2023 1,732 3 View

What is wrong with Residual plasmid testing using qPCR in AAV products?

I am testing the residual plasmid quantity using qPCR in AAV gene therapy products. The aim is to quantify the level of residual plasmid in AAV products. I prepared standards using diluted...

29 March 2023 2,701 2 View

What is the best strategy to perform huge ratio of dilution?

We are testing virus titer, and have to dilute the virus stock at 1:10,000 (Dilution A) and 1:20,000 (Dilution B) to run qPCR as templates. Then convert the diluted titer to dilution corrected...

16 March 2023 8,098 4 View

Can 0.1% Triton X-100 or NP40 break nuclear membrane after long time incubation?

I am going to isolate nuclear protein from ~30 tissues. The lysis buffer contains 10mM NaCl and 0.1% Triton X-100 which is reported with the ability to lyse cell membrane only (but not nuclear...

30 June 2016 7,696 3 View

Dynabeads vs Agarose Beads vs MicroBeads, what is the best choice?

Actually here I have 2 questions, one is for IP experiments, and another is for cell isolation. And both are about the choice of beads. Question 1: What bead is better for IP? Dynabead from...

28 April 2015 4,232 4 View

Can anybody recommend good antibodies against human Dnmt1, Dnmt3a, and Dnm3b for western blot?

Thank you so much.

25 June 2014 1,674 9 View

Is it possible to collect thioglycollate induced peritoneal macrophage and bone marrow derived macrophage from the same mouse?

We don't have enough knockout mice, so I want to collect both peritoneal macrophage and bone marrow derived macrophage (BMDM) from the same mouse. But I don't know whether thioglycollate has any...

01 October 2013 8,872 10 View

Immunoprecipitatation (IP)/Co-IP of acetylated nuclear protein?

I am doing IP of my POI, a nuclear protein. Ac-POI is a kind of transcriptional factor. I have several questions. 1) How to protect acetylation modification of POI? Protease inhibitor cocktail...

13 July 2013 4,510 2 View

What is the best cell model for inflammation in sepsis?

I am working with mouse Immunol cells. RAW264.7? Bone marrow derived macrophages? Peritoneal macrophages? or Spleen cells? or blood leukocytes?Thanks.

28 March 2013 1,893 0 View

How to prepare the solution of palmitic acid or stearic acid?

Does anybody has a detailed protocol?

11 January 2013 6,157 14 View