Can the same sample with a negative result in conventional PCR, give a positive result with real time PCR?

14 October 2014 364 5 View

Does anyone know what genus and/or species of Cladocera this is? Or if it is Cladocera?

I found it with a phytoplankton net in the Oslo fjord, right near the shore. It's quite small compared to most of the Daphnia I've seen. About the size of a large (or two tiny) Ceratium longipes...

11 August 2014 4,361 19 View

QPCR: reference gene stable under hypoxic conditions?

I'm trying to compare mRNA expression levels of my gene of interest in cells at normoxic vs. hypoxic (i.e. 1% O2) conditions. Usually I would use GAPDH as my reference gene but it seems that GAPDH...

31 March 2014 5,003 48 View

How many units of enzyme should I use for enzymatic deglycosylation of pancreatic ECM?

I am trying to establish a protocol for enzymatic deglycosylation of decellularized porcine pancreatic tissue. We want to optimize our LC-MS/MS analysis of the ECM. We have 4 different enzymes...

16 March 2014 1,046 2 View

Identified vs anonymous survey answers

Hi. When delicate topics like perceived social norms regarding immigration, violence, racism etc are measured and political affiliation needs to be considered, what is the opinion on identified...

01 January 1970 8,181 4 View

Can anybody recommend what measure might predict how open children are too difference?

The study is based of first impressions of autistic children.

01 January 1970 7,233 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View