Can the same sample with a negative result in conventional PCR, give a positive result with real time PCR?

14 October 2014 364 5 View

Does anyone know what genus and/or species of Cladocera this is? Or if it is Cladocera?

I found it with a phytoplankton net in the Oslo fjord, right near the shore. It's quite small compared to most of the Daphnia I've seen. About the size of a large (or two tiny) Ceratium longipes...

11 August 2014 4,361 19 View

QPCR: reference gene stable under hypoxic conditions?

I'm trying to compare mRNA expression levels of my gene of interest in cells at normoxic vs. hypoxic (i.e. 1% O2) conditions. Usually I would use GAPDH as my reference gene but it seems that GAPDH...

31 March 2014 5,003 48 View

How many units of enzyme should I use for enzymatic deglycosylation of pancreatic ECM?

I am trying to establish a protocol for enzymatic deglycosylation of decellularized porcine pancreatic tissue. We want to optimize our LC-MS/MS analysis of the ECM. We have 4 different enzymes...

16 March 2014 1,046 2 View

Identified vs anonymous survey answers

Hi. When delicate topics like perceived social norms regarding immigration, violence, racism etc are measured and political affiliation needs to be considered, what is the opinion on identified...

01 January 1970 8,181 4 View

Can anybody recommend what measure might predict how open children are too difference?

The study is based of first impressions of autistic children.

01 January 1970 7,233 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View