Where can I order dig-lablled RNA probes of in situ hybridization?

More Carina Dubs's questions See All

Shoul I discount the salt mass from the buffer while calculating the hydrolysis degree?

We are hydrolyzing a sample and the medium we are using is a buffer solution with a considerable amount of salt. When I am calculating the protein content from the sample obtained at the end of...

26 June 2023 5,133 1 View

Data from manipulation check not normally distributed - ist that a problem?

Hello all! As part of my master's thesis, I did an experiment. I now have 5 groups with n=45 participants each. When I look at the data for the manipulation checks, they are not normally...

12 September 2022 7,782 14 View

How to best establish change in one group pre-/post design with small sample?

Hello everyone. I am planning a one-group pre-/post test design (I am aware of the limitations, including a control group is unfortuntately not feasible) and want to measure whether var 1...

20 August 2021 1,881 4 View

Could someone please help me compute a variable for household equivalent income in SPSS?

I would like to create a variable, taking into account the person's income, number of children, and the total number of people living in one household. The idea would be that if the respondent...

13 April 2021 2,606 3 View

How to deal with non-normal data AND composites that were calculated in different ways?

Hi. I am dealing with data that shows negative skewness and variables that were calculated in different ways: Some of the variables´ composites were calculated from the mean, but others had...

01 April 2021 5,344 4 View

What can be detected automatically in a hall scan (e.g. of a production environment) with current data analysis methods?

Hall scan data can consist of point clouds, (3D) images and videos... I'm curious if you know about any projects or ongoing research in this area. The aim of the object detection could be the...

11 August 2020 7,813 3 View

MLM: Adding group means when only using grand mean (centering)?

Hi. I am currently working on a multilevel analysis and have decided to use the grand mean (and grand mean centering) instead of the group mean (centering). I wonder though whether I have to add...

20 April 2019 5,597 2 View

Daphnia immobilisation test: negative controls are fine after 24h, but 100% immobilised after 48h?

I am currently doing the daphnia acute immobilisation test and am trying to find out why our negative controls are fine after 24 h, but immobilised after 48 h. O2 levels and pH do not seem to be...

16 April 2018 2,816 5 View

Which are the best checklists for quality assessment of cross-sectional and cohort observational studies in a systematic review?

STROBE checklists seem to be used frequently. However, it might not be the most appropriate tool as the original purpose was to serve as a guideline for reporting observational research and not...

18 November 2016 6,550 43 View

Do you know of any systematic reviews on family nursing, reporting on families and children with complex health care needs, in a primary care setting?

I am a master degree student in clinical nursing, working on a systematic review for my final exam. Advise and thoughts from experts in the field are an important part of my search strategy. In...

16 January 2016 2,449 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

Sharmin Hasan

Hi Carina,

https://www.jenabioscience.com/images/741d0cd7d0/Biotin_DIG_Nuleotides.pdf.

This link may help. Best wishes!

Wenting Li

Hi Carina，

I had ordered the probe of ISH in EXIQON and the efficiency was very well. And you can choose the label, such as 5' DIG, 3'DIG or 5' and 3' DIG.

Best wishes!

Tando Maduna

Although I prefer to make my own probes, I'd recommend ACD https://acdbio.com/. They are very helpful and approachable should you have questions. You can also try to access their training webinars just to get started.

All the best!