I am culturing my human keratinocytes (isolated from hair follicles) in a serum-free EpiLife medium which is low calcium, however for reasons I need to increase the calcium level to the standard. What kind of Calcium I can use and how much to add to 500ml media?
Hi Hassan,
There are lots of calcium experts in your university. I would recommend that you visit Dr Jason Bruce, Dr Katharine Dibb, or Professor Andrew Trafford and ask for help (amongst several others). They are all really nice people, who I am sure will help you. It might be easier to talk to them directly than advise you here on Researchgate. You probably need to increase the calcium to approximately 1.5 - 1.8 mM by adding a calcium salt (such as calcium chloride) to you medium.
Best wishes,
Martin
I use 0.07 mM CaCl2 for my HEKa and neonatal foreskin HEK cells as the standard growth concentrations and then if I do decide to differentiate them I will incubate them in 0.03 mM Ca for 24 hours and then switch to the high calcium concentration of 1.2 mM, which from what I've seen is standard. I always buy medium without Ca and then add as much CaCl2 as I need for each condition. I also use serum free media. Hope this helps.
- Badges
- Science topic