Hi everyone,
We have synthetized a FQ probe, 12nt ssDNA oligonucleotides modified with FAM/BHQ1 at the two ends respectively. The probe was dissolved by DNase/RNase-free water with 10 μM and preserved at -80℃. When the probe used, we thawed and diluted it. Now, we wonder whether it is appropriate to dissolve the probe with water. If not, what buffer and pH should be used to dissolve the probe, and what concentration should be used for preservation without damage.