Lin Huang

6 Questions 10 Answers 0 Followers

Questions related from Lin Huang

How can I increase the ssDNA yields in asymmetric RT-RPA amplification ?

Hello everyone， I am developing asymmetric RT-RPA amplification to obtain the ssDNA products. The outputs of the ssDNA still are very low although I tried to various ratios of excessive and...

16 April 2024 5,496 3 View

Any advice for reaction buffer of AuNP conjugation with PAMAM-SH?

I would like to ask everyone to recommend a buffer solution suitable for the reaction between AuNP(15nm) and PAMAM4.0-SH. Thanks!

25 June 2023 2,887 0 View

Any advice for reaction buffer of AuNP conjugation with PAMAM-SH?

I would like to ask everyone to recommend a buffer solution suitable for the reaction between AuNP(15nm) and PAMAM4.0-SH,thanks!

16 June 2023 9,227 0 View

What buffer was used to dissolve synthetic FQ probe?

Hi everyone， We have synthetized a FQ probe, 12nt ssDNA oligonucleotides modified with FAM/BHQ1 at the two ends respectively. The probe was dissolved by DNase/RNase-free water with 10 μM and...

11 November 2022 3,189 0 View

Why did the protein containing MBP-tag aggregated and precipitated when cleaved by Tev protease?

Hi，everyone， I am purifying a protein containing the contrust: N terminal-10×His+MBP+Tev site+protein-C terminal. The binding buffer is 50mM Tris-HCl, pH7.5, 500mM NaCl,10mM imidazole, 5%glycerin,...

20 June 2022 6,927 3 View

Can Cas9 and gRNA be injected into cells directly?

I recently inserted a GFP fragment into mammalian cells with CRISPR/Cas9 , tagging the GFP to the target gene using homologous recombination; I am wondering 1) if Cas9 protein and gRNA would be...

01 January 1970 5,245 4 View