I tried using Eugon broth, but failed to cultivate. Any suggestions?
Try using trypticase arginine serine broth infused with 4% hoof powder
can any body suggest the best and cheap adjuvant that should be used as im using alum for my work it producing severe cellulitits even though it enhances immunity.
01 February 2015 9,576 19 View
i have lots of question regarding the working ability of rabies vaccine as it is highly neurotrophic but how it produce the immune effect through out the body?
11 December 2014 8,248 2 View
I'm doing antibiotic sensitivity test for the mastitis organisms but in feed back some reporting the same problem even after use of the specifically listed drugs. Can anybody suggest the proper...
10 November 2014 557 6 View
I'm working on mastitis organisms, even the general causative microbes are also taking a longer time for culturing so I'm waiting for good suggestions.
09 October 2014 1,428 1 View
For examination in a laboratory.
04 May 2014 5,113 11 View
I would like to read some information to improve my knowledge on adjuvants.
03 April 2014 7,714 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View