In addition to the above criteria, the cell line should be suitable for RNAi and gene expression studies, hence easy to transfect and to infect.
Here are some breast cancer cell lines that we have made transfection kits for and validated with siRNA-based gene silencing (i.e. RNAi):
- MCF-7 (https://altogen.com/product/mcf-7-transfection-reagent-breast-cancer-htb-22/)
- BT-20 (https://altogen.com/product/bt-20-transfection-reagent-breast-carcinoma-cells/)
- MDA-MB (https://altogen.com/product/mda-mb-transfection-reagent-breast-adenocarcinoma/)
- SK-BR3 (https://altogen.com/product/sk-br3-transfection-reagent-breast-adenocarcinoma/)
- 4T1 (https://altogen.com/product/4t1-transfection-reagent-breast-cancer-crl-2539/)
4T1 is of murine origin, so it's not going to be all that good for studies focused on humans.
We work with MCF7 (low PR expression) and T47D (high PR expression). We used to use them as models when targeting PR with siRNAs. We use them for a lot of molecular biology experiments these days. They both transfect quite well and have strong RNAi machinery expression. HeLa cells are obviously a good control cell line to use, as they have a very robust RNAi pathway. These cell lines are all well characterized and commonly used, but aside from HeLa, MCF7 is perhaps one of the most common. Let me know if I can answer any questions about these cell lines.
MCF-7 and MDA-MB231, are well-known as estrogen receptor positive and negative Invasive ductal carcinoma, respectively.
You can find a list of cell lines for breast cancer in the Wikipedia:
http://en.wikipedia.org/wiki/Breast_cancer
Good luck,
MCF7 is a very commonly used breast cancer cell line. The same is commonly used as a positive control for a number of experimental setups and are easy to both, transfect and infect.
also use this source
http://www.lgcstandards-atcc.org/ATCCCulturesandProducts/CellBiology/CellLinesandHybridomas/tabid/981/Default.aspx
Thanks so much for your valuable contributions.
Aamir: You are absolutely right, but we would like to establish breast cancer cell lines as a cell model for RNAi experiments (essentially as described by Keith Gagnon above), rather than to investigate molecular mechanisms in cancer biology. Thanks for your suggested cell lines.
you should notice about malignancy rate or other applications
from poor prognosis too low aggressive cell lines are different
and ER/PR/Her2 is important in your study
Luminal A ER+, PR+/–, HER2– Ki67 low, endocrine responsive, often chemotherapy responsive MCF-7, T47D, SUM185
Luminal B ER+, PR+/–, HER2+ Ki67 high, usually endocrine responsive, variable to chemotherapy. HER2+ are BT474, ZR-75
trastusumab responsive
Basal ER–, PR–, HER2– EGFR+ and/or cytokeratin 5/6+, Ki67 high, endocrine nonresponsive, often MDA-MB-468, SUM190
chemotherapy responsive
Claudin-low ER–, PR–, HER2– Ki67, E-cadherin, claudin-3, claudinin-4 and claudinin-7 low. Intermediate response BT549, MDA-MB-231,
to chemotherapy Hs578T, SUM1315
HER2 ER–, PR–, HER2+ Ki67 high, trastusumab responsive, chemotherapy responsive SKBR3, MDA-MB-453
Dear Colleagues,
Could you please provide me with the following breast cancer cell lines:
MCF-7, T47D, SUM185, MDA-MB-468, SUM190, MDA-MB-231, SUM1315. What I am looking for is cell line s for non inflammatory breast cancer and inflammatory breast cancer with HER2+. Many thanks.
Dr. Abdelbaset Elasbali
Senior Research Fellow
The Centre for Healthcare Science Research
Manchester Metropolitan University
John Dalton Building
Chester St.
Manchester M1 5GD
Tel 0044 161 247 3366
Here are some breast cancer cell lines that we have made transfection kits for and validated with siRNA-based gene silencing (i.e. RNAi):
- MCF-7 (https://altogen.com/product/mcf-7-transfection-reagent-breast-cancer-htb-22/)
- BT-20 (https://altogen.com/product/bt-20-transfection-reagent-breast-carcinoma-cells/)
- MDA-MB (https://altogen.com/product/mda-mb-transfection-reagent-breast-adenocarcinoma/)
- SK-BR3 (https://altogen.com/product/sk-br3-transfection-reagent-breast-adenocarcinoma/)
- 4T1 (https://altogen.com/product/4t1-transfection-reagent-breast-cancer-crl-2539/)
4T1 is of murine origin, so it's not going to be all that good for studies focused on humans.
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