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Questions related from Peter Kloehn
Whole genome sequencing and genome-wide association studies (GWAS) clearly demonstrate the complexity of common diseases and in the majority of cases risk cannot be defined by a single gene...
07 July 2014 5,768 2 View
The early immune response tends to produce polyspecific antibodies, i.e. antibodies that recognize various epitopes and IgM antibodies are a classical example. However, during affinity maturation...
07 July 2014 9,893 2 View
We aim to assemble 6 PCR fragments (300-400 bp) by Gibson assembly. We assembled and PCR amplified the first 3 and last 3 fragments with no problems. However, the assembly of the two amplicons to...
02 February 2013 9,239 14 View
In addition to the above criteria, the cell line should be suitable for RNAi and gene expression studies, hence easy to transfect and to infect.
02 February 2013 8,578 10 View
What is the best method for viral packaging of vectors harbouring large coding sequences (>8kb)? Our efforts to package a 9 kb coding sequence with a lentiviral vector failed, whilst we obtained...
01 January 2013 6,845 12 View
