Cells will have lower viability upon treatment of high concentration of fatty acids, particularly with Palmitic acid. Does anyone know any sensitive biomarkers to detect the lipotoxicity process?
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I am seeking advice on the matrix and solvent conditions.
29 May 2024 2,360 0 View
I found it very difficult to define it precisely. Microspore and megaspore of ferns or seed plants are sexual spore? Because the fertilization happened for a long time before the formation of...
27 April 2024 3,077 0 View
Hi all, I'm Dabin Xie from Zhincai Tech, a small research-driving company in China. I'm currently trying to modify glass with a single layer of silane whether hydrophobic or hydrophilic, like...
08 April 2024 2,628 4 View
How to solve this problem when Aspen economic evaluation was performed? It said "Temperature difference is insufficient to perform the heat transfer."
06 April 2024 7,291 3 View
If I want numerical simulation software to simulate superconducting magnetic shielding, which software is better for simulating superconducting magnetic fields?
25 March 2024 5,589 1 View
I've encountered an issue following an LR reaction intended to construct a gene within a pENTR-3C (KanR) vector and combine it with a pCDH (AmpR) vector. Despite expectations, the protein was not...
14 February 2024 3,502 0 View
I tried to make a moive of protein structure in PyMOL. However, when I stored the movie for future use, the PyMOL reminded that the encoders were not installed. How to install these encoders in...
29 October 2023 5,129 2 View
I have performed the 3C-qPCR assay and designed the primers of interested region, but I do not have BAC clone as control. How should I normalize the prime efficiency and background noise?
16 October 2023 4,387 0 View
In the XRD experimental analysis of polycrystalline TiN films, are TiN (111) and TiN (222) different crystal planes? Or are they the result of different diffraction levels of the same crystal...
26 September 2023 3,580 5 View
If a protein was designed as a closed cyclic protein (N-and C-terminus closed after reaction), can PyMOL show this structure? Because in PyMOL, the sequence is always linear, so how the closed...
17 September 2023 2,886 4 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View