Cells will have lower viability upon treatment of high concentration of fatty acids, particularly with Palmitic acid. Does anyone know any sensitive biomarkers to detect the lipotoxicity process?
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Hi all, I am now working with ITS2 sequencing library preparation. I had observed that after introducing adapters, linkers and barcodes to a primer, the indexed primers have a much lower...
27 January 2021 5,045 3 View
I understand several normal DNA intercalators, such as ethidium bromide, acridine, SYBR et al. would emit fluorescence. Is there any kind of DNA intercalator that do not have fluorescence?
25 December 2020 688 3 View
I learned that in order to interpret the centrality indices, the CS coefficients resulted from a bootnet test are preferably above 0.5 and above 0.25 is acceptable. In my network, the CS...
29 October 2020 3,545 3 View
I've been working on the network analysis of adolescent depressive symptoms. I've estimated the network, the centrality indices and network stability using packages qgraph and bootnet. Then I want...
22 October 2020 5,591 3 View
25 September 2020 9,936 2 View
These days I wanted to run the Lagranto model to track water vapor transport path. But I got some trouble in installing an available version of Lagranto model. I attempted to use "conda install -c...
04 July 2020 5,417 4 View
Hi, I am trying to run a vumat subroutine, but there is some abaqus-fortran linking issue. I use abaqus 6146 Micrasoft visual studio 14.0 parallel studio XE 2016 I added these command in my .bat...
25 February 2020 5,698 2 View
In digital health domain, is the academic research that leads the development of the industry OR is the industry development that impacts the associated academic research ?
10 June 2019 5,214 6 View
When I titrated fluorescence probe by Protein A alone or by Protein B alone, it do not should any binding signals, mP do not change. However, when I titrated the same fluorescence probe by...
18 March 2019 2,468 3 View
I would like to know information about esiRNA form Sigma for the following points: 1. transfection protocol; 2. knockdown efficiency; 3. off target effects; 4. toxicity. Anyone would like to share...
31 May 2018 9,661 2 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...
27 February 2021 3,882 3 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...
25 February 2021 2,225 5 View
The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...
23 February 2021 2,049 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View