I'm interested in groups of compound which are the most common in acetone extracts
@Dr. Sharangouda J. Patil, @Chaitanya Mvnl, many thanks for your answers.
Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...
26 January 2021 4,071 3 View
I need to find metals, plastics or metal alloys that have a specific Vickers scale hardness scores. I'm not aware of any existing web sites and tools that could aid me in searching for this data,...
06 August 2020 4,842 4 View
Hello, We're conducting quantitative research as a part of this semester, and the analysis method of choice is multivariate linear regression. Right now, we're checking for the fulfillment of...
03 November 2019 2,694 3 View
In general, sex ratio is expressed as the ratio of males to females in a population, ie number of males/number of females (*100). However, when the number of females in (sub)population is 0...
18 August 2019 1,856 28 View
Hi, I'm currently having problems with Western blots using the following antibodies from Cell Signaling: Phospho-Stat3 (Tyr705) STAT3 (total) Phospho-Stat5 (Tyr694) STAT5 (total) They seem to...
11 August 2019 3,463 4 View
Since using PLE carriers can be excited to the bottom of the conduction band, where they kinetic energy is zero it should be less probable to detect luminescence coming from carriers influenced by...
10 July 2019 3,335 3 View
Can I transfect HEK293 cells using siRNA purchased form Dharmacon and use Lipofectamine reagent instead of DharmaFECT? I have been using electroporation for the transfection, but it seems like HEK...
21 January 2019 2,884 3 View
Hi, I want to do an overlap extension PCR (OE-PCR) using a PCR-amplified plasmid backbone and a PCR-amplified insert. The OE-PCR shall add the insert into the plasmid similar to this...
12 July 2018 1,976 3 View
I am from Czechia (in past Czechoslovakia). I do research to check if the problems in choosing a field of study (measurred by CDDQ) are related to MBTI typology dimensions. I know this method is...
28 April 2018 9,264 1 View
We are looking into liquid handling systems for pipetting qPCR plates (384 well) and for NGS library preps (amplicon seq). There are many machines on the market that are able to do that (Biomek...
25 February 2018 8,701 5 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I looking for ferro calcium production process
03 March 2021 1,755 4 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...
24 February 2021 1,265 6 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View