Is there a dataset available on actual individual person usage of OS? If not what is the best way to create it?

I want to create a N.N which can learn person OS usage based on their use of application and data related to specific application's background time, usage frequency, usage time of the application...

07 December 2020 1,252 4 View

What is causing the delay in expression of EGFP in HEK293T transient transfection with pAAV plasmid?

I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...

15 January 2020 4,057 1 View

How can I interpret the negative value of regression coefficient in logistic regression??

I am running a logistic regression by using dichotomous dependent variable and five independent variable. I found one of the independent variable is getting -ve regression coefficient. 1) What...

07 November 2017 4,464 9 View

How Gray level co-occurrence matrix or GLCM calculated for an image is implemented on that image for texture feature extraction?

Information such as contrast, entropy, dissimilarity index and variance are computed to know the properties of any image, but how these values are used to for further classification or feature...

12 October 2017 4,978 3 View

Entering the field of Data Science as a developer

Being a python programmer, I have decided to get in the field of data science and later on AI and Machine Learning. I have already brushed up the knowledge of statistics and other python libraries...

23 August 2017 5,999 13 View

What are the assay I should use for insulin receptor activation on NIT cell lines?

We have NIT cell lines, what are the assay should I use for checking insulin receptor activation ?

10 May 2017 9,836 1 View

Is atmospheric correction mandatory for chlorophyll estimation from Landsat images and which is best method of atmospheric correction?

I am trying to estimate chlorophyll-a by using Landsat/ MODIS image. So I want to know atmospheric correction for the above analysis is required or not? There is so much technique for atmospheric...

08 March 2016 9,488 2 View

How can I calculate the load of a virtual machine?

In a virtual machine there is a migration. Let's suppose i want to select a virtual machine, based on it's current load, and make a decision based on the current load to migrate virtual machine....

06 September 2013 9,969 18 View

How to measure OFDM PAPR in Matlab Simulink?

01 January 1970 7,579 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View