What assay I should perform for insulin receptor activation?

More Harshit Jadav's questions See All

Plant tissue culture: ammonium sulfate instead of ammonium nitrate? what concentration?

Plant tissue culture: ammonium sulfate instead of ammonium nitrate? what concentration? how to prepare stock 1 solution for MS media using ammonium sulfate?

07 May 2023 4,177 3 View

Can I use DEAE Sepharose in place of DEAE Cellulose?

I have been trying to purify Amyloid Beta peptide using ion exchange chromatography. In the paper I am referring to, they have used DEAE Cellulose in their protocol. I have been using Q-Sepharose...

15 January 2023 2,501 0 View

How to reduce noise in OCP and EIS study using Potentiostat?

Dear all, I have been doing trials for accessing corrosion properties of steel rebar in chloride environment using Potentiostat, wherein my working electrode is steel rebar, reference electrode is...

15 November 2022 8,797 0 View

Dear all I wanted to Ask is there are any traditional method for isolation of T Cells?

is there any traditional method to check T cell levels or can we isolate T-cells to check Th17 cell levels using specific markers? I am a little stuck here. Just to clarify I don't want to use...

20 September 2022 1,266 1 View

Protocol for induction of shooting from callus chickpea?

I get callus from chickpea genotype but i want to know which types of media and hormones concentration required for generation of shoot from callus

30 August 2022 637 0 View

Hi everyone. So I'm trying to locate nanoparticles inside plant tissues. Is there a technique I could use that can detect them without sectioning?

I need a rapid protocol for the detection of any spectral signature of nanoparticles inside plant tissues without necessarily sectioning the tissue. Is it possible?

28 August 2022 2,370 0 View

What is best protocol for estimation of proline, MDA content Catalase and SOD for plant leaves ?

02 June 2022 2,229 2 View

What is best method of surface sterilization of explant in tissue culture ? and how to minimize fungal contamination in tissue culture ?

what is best method of surface sterilization of explant in tissue culture ? and how to minimize fungal contamination in tissue culture ? Can I use Fungicide for surface sterilization of explant...

02 May 2022 8,709 4 View

How to use Case study method in Design Management research?

I'm conducting research to understand the design management practices in early stage start-ups. I 'm taking a action research and case study method as my approach. Would like to connect with...

14 February 2022 7,613 0 View

What type of plasticity model and its data for wood material should be input in ABAQUS software?

I am an M.Tech structural engineering student working on the project ' Numerical analysis of Kath-kuni architecture ( a common masonry typology ) in Himachal Pradesh region of India subjected to...

11 January 2022 1,154 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Souhad El Akoum

Hi Harshit,

you can look, by westernblot, phosphorylation of Akt, P38 or look at the dimerization of the insulin receptors by doing native western blot and maybe using crosslinker

Biswajit Majumder

You can follow the following links... Thanks

https://www.nature.com/articles/srep07911

http://gradworks.umi.com/37/06/3706555.html

https://books.google.co.in/books?id=-lkyAErKCdgC&pg=PA64&lpg=PA64&dq=insulin+receptor+activation+acessy&source=bl&ots=gN2gruG4RN&sig=8qPyNgtFFvpjLuDCNTKgrDKoVPs&hl=te&sa=X&ved=0ahUKEwiXyMTh6cjTAhUiS48KHWrECOY4ChDoAQhCMAo#v=onepage&q=insulin%20receptor%20activation%20acessy&f=false

http://www.sciencedirect.com/science/article/pii/S0003269785799927