Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

What is the difference between PBE and PBEsol functional?

Hello, I am a 3rd year chemistry student and I'm writing my project report on computational chemistry. does anyone know how PBEsol improves upon the PBE functional? Thanks in advance

19 February 2021 7,068 4 View

Can someone help me with this error that I am getting while estimating a VAR model in R?

I am using annual time series data to estimate a VAR model in R. However, when I run the command to find the optimal lag length, I get the following error: Fehler in VARselect(data, lag.max = 10,...

18 February 2021 9,647 3 View

Post evaluation of close range photogrammetry experiments ?

I am performing a simple close range photogrammetry test and comparing outcomes of photogrammetry tools such as VisualSFM, Meshroom, Colmap etc. Kindly advice, what post evaluation tests/ criteria...

13 February 2021 6,410 3 View

In your experience what is the difference between G.652D and G.655D optical fiber. In field and in lab?

In our current era there is a big confusion about the usage of G.652 and G.655 optical fiber cable. Theoretically G.655 is much better than G.652 but the operator feel that G.652 is giving almost...

11 February 2021 4,006 8 View

How many dilutions are good enough for TCOD50 of SARS-CoV-2?

I started with 10e-1 till 10e-10. But when I searched for online calculator they mostly consider 5 dilutions. Is there a reason behind it or I should make less dilutions?

11 February 2021 5,839 3 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View

How to convert ng/ul of DNA concentration to pmol/ul ?

Dear researcher, After amplifying my target gene (1900bp) into the required cDNA by PCR,>cut specific gel bands and purify the gel and the concentration was 30ng/ul. Next, performed A-Tailing...

21 January 2021 2,671 3 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

Best way to optimize species-specific primers? Can I use a temp gradient to test a Ta where I will get target (and no non-target) amplification?

Hello, I am testing different species-specific primer sets. I have two targets (two species, but same gene target). I am trying to select the best primers -- one that will amplify the target...

18 January 2021 1,981 6 View

How can i perform gene overexpression study in vivo? in sheep or mice model?

I am planning to transfect/infect a target gene directly into sheep or mice body to study its effect. need guidance please

03 December 2020 434 4 View

Only my PCR primers are not working?

I am working on an undergraduate project within a group. The topic is to identify carbapenamase resistnace gene in K.pneumoniae and E.coli. The target genes are OXA-48, KPC, IMP and VIM. We are...

26 November 2020 6,992 5 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

What is the difference between PET28a(+) 6595bp and PET28a(+) 5369bp?

I am doing restriction cloning, and I want to choose a restriction enzyme and clone my target gene into a PET28a protein expression vector. I'm confused about which of the two vectors to use since...

12 November 2020 5,378 3 View

CRISPR-Cas9: Are there any alternatives to the use of fluorescent PCR and capillary electrophoresis?

We are trying to find alternatives to the use of fluorescent tagged primers and capillary electrophoresis to access somatic activity of the target gene. Thank you!

25 September 2020 4,761 3 View

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How can I know the expression level of common tumor targeting receptors on cancer cell lines? For example the cell lines and receptors I mentioned in the question. Thanks a lot.

31 August 2020 5,347 3 View