I would like to know for how long can I store X Gal and IPTG solutions at -20°C and use it to select transformed E coli without any loss of efficiency?
1M stock solution of IPTG is stable for 6 months at -20. X-Gal is also stable for 6 months at -20C, however as you may use x-gal vial multiple times it is better to restock it after every 2 to 3 months. Note- X-gal is hygroscopic