Pedro Brito

3 Questions 1 Answers 0 Followers

Questions related from Pedro Brito

What are X Gal and IPTG storage conditions?

I would like to know for how long can I store X Gal and IPTG solutions at -20°C and use it to select transformed E coli without any loss of efficiency?

12 December 2018 1,253 3 View

How long it takes for E. coli cells to reach OD 0.5 at 600nm?

I'm going to follow Inoue's protocol for competent E. coli cells. It says that I should incubate it in 250ml SOB medium 18°C-22°C at moderate shaking. Then, I should read the OD600 in the next...

10 October 2018 8,092 9 View

How long it takes for E. coli cells to reach OD 0.5 at 600nm?

I'm going to follow Inoue's protocol for competent E. coli cells. It says that I should incubate it in 250ml SOB medium 18°C-22°C at moderate shaking. Then, I should read the OD600 in the next...

08 October 2018 5,206 8 View