Pedro Brito

2 Questions 1 Answers 0 Followers

Questions related from Pedro Brito

How long it takes for E. coli cells to reach OD 0.5 at 600nm?

I'm going to follow Inoue's protocol for competent E. coli cells. It says that I should incubate it in 250ml SOB medium 18°C-22°C at moderate shaking. Then, I should read the OD600 in the next...

10 October 2018 8,039 9 View

How long it takes for E. coli cells to reach OD 0.5 at 600nm?

I'm going to follow Inoue's protocol for competent E. coli cells. It says that I should incubate it in 250ml SOB medium 18°C-22°C at moderate shaking. Then, I should read the OD600 in the next...

08 October 2018 5,122 8 View