Hello everyone,
I am doing imaging experiments on samples immersed in 400uL media in an 8-well dish. The goal is to image z-stacks of multiple positions through time (xyzt, mark and scan). We have a Leica DMI6000 with the Leica Application Suite X installed.
I have an issue where the z-stack changes over time, so gradually my samples in each position aren't imaged in the correct z-dimension range. We have tried using autofocus, but the fluctuations may be too small for it to work?
We would like to slow down the speed of the stage as it changes positions. We think the z-dimension fluctuations are arising from too rapid stage movement that causes the liquid in the wells to affect the z-dimension. Does anyone know how to adjust this setting?
Hi Sebastian Schmitt
, sorry for the delay in my reply! I have tried using the AFC but the drift still seems to happen...
Hi Kendra,
You can adjust the speed and the acceleration of the stage in the LAS X Hardware Configurator which is a software normally installed next to LAS X.
You have to select ''Configure'' in the setup tab and ''XY stage'' in the component tab. Then you will see the name and article number of your stage, select ''Fine tuning'' to access to the settings.
Maxence
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