I found these organisms in the roots of 45 days old Red Kidney bean plants. They were in huge amount and scattered or in chain. Would you please identify these organisms?
Thanking you all
Is it possible that you are looking at the early stages of a Rhizobium infection?
It probably looks like a Rhizobium infection, perhaps a thin LS would help identifying which symbiotic organism it is. It is probably a nitrogen fixing organism trying its primary mode to colonize the roots of the legumes (the pea plant family). A selective growth nutrient media can help in growing the organism to isolate and further study the features of the same.
How far is the image magnification ?, because Rhizobium is very small, if a high magnification is, then could be Rhizobium. Is the sample that you got, has some sort of nodes in the root ?, sometimes these knots are of a red color who indicates that they are actively present. If you need to know this species, only extracts their DNA sequence and then compare in a database on the Internet.
Probably these are mycorrhizal fungi showing vesicles filled with phosphate granules
Dear Dubey,
these are not mycorrhizal fungi .... and its neither vesicles. Arbuscular Mycorrhizal vesicles does not occur in chains.....Phosphate granules are not found in vesicles but they are present in hyphae and Arbuscules. of Am fungi
Dear Partha Pratim Dhar,
Probably these are some other endophytes and not mycorrhizal fungi..try to isolate and culture them them on media..and observe the on hyphal growth pattern, branching, septation etc.. i feel that this might be one species of the Dark septate endophytes ....
for more information visit the http://www.hindawi.com/journals/jb/2014/173125/fig1/
Rhizobium is seen associated with almost all fabaceae members roots and they fix Nitrogen.
I am highly glad and feel inspired to see all the helpful comments. I owe my gratefulness to you all. But I have no experience on Rhizobium. So far I know, they are N-fixing, that is all. Would you please provide me some protocols to isolate and culture rhizobium. I think it is a new observation for me.
Prof. Kris Joseph Mahoney, Prof. Jimmy John Lilly, Prof. Andrei Gonzales I. , Prof. R.C. Dubey , Prof. D Souza, Prof. Narayanan Nair Mohanan, my best salute to you for amazing and thrilling comments.
Prof. Andrei Gonzales I. :
I took all the pictures at 40 magnification. Only one is at 20 magnification. Soil temperature is around 15-17C.
Thank you all my distinguished colleagues.
Best Regards
ResearchGate tweeted your question (https://twitter.com/ResearchGate/status/542986886839808000) - congratulations!
Your welcome, Like I said Rhizobium is too small, so I suggest that it is not Rhizobium, I'm not microbiologist, but I Agree with James DSouza that is posible it have to be something with Fungi species; please add on your topics Microbiology, Fungi taxonomy and molecular DNA identification, maybe this people could give us more information, also if you can give us more information about the plant about the species.
Dear Partha Pratim Dhar
In the laboratory, rhizobia are grown on a special medium called yeast-mannitol agar
(YMA). They are grouped in two main genera—the fast-growing Rhizobium species and the slow-growing Bradyrhizobium species. When cultured on YMA, the Rhizobium
species produce visible growth in two to three days. They produce an acid growth reaction, which can be detected by adding a pH indicator, bromthymol blue (BTB), to the medium.Rhizobia isolated from pea, bean, clover, alfalfa, chickpea, and leucaena are all fastgrowers. The soybean and cowpea rhizobia are slowgrowers.
best wishes
thank you a lot, D. Souza. I request you earnestly to send me a copy of your article in the http://www.hindawi.com/journals/jb/2014/173125/fig1/
And don't forget to give me your email, please. My email is [email protected]
These are endophytes, try to isolate them on synthetic media. If the plants wer healthy than others, this may be the great achivement. you can also make a pot culture studyWhich Stain you used during the study
Dear Mir Hasan,
The plant are 45 days old and they are not only healthy but also growing well. Plant is leguminous and it is Red kidney bean. Thank you for suggestion. Could you please give me some literature regarding isolation and identification?
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